FastQCFastQC Report
Mon 27 Feb 2023
SRR3127808.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127808.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757513
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88991.1747653175589066No Hit
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG30640.4044815072480604No Hit
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG28600.37755127634773267No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC26640.35167713293369224No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA12930.17069013997119523No Hit
AGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT9110.12026196250097358No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC7840.10349657365616168No Hit
GGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT7790.10283651897723207No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA750.087.932794
CGTTTTT28850.086.556631
CTAACGG401.0149961E-982.4533541
CGTAGGG1900.081.817172
AGGGTAC3250.080.975865
TAGGGCA4250.080.912954
TACGGGT700.080.754613
TATTAGG650.079.7351151
AGTACGG1200.078.5270161
GGATACG600.078.324868
GAATAGG4050.077.945331
GTACGGG2250.077.464612
TAAACGG551.8189894E-1277.099241
TAGCGGG2900.076.34562
AGGGCAT7050.075.325195
TGACGGG2650.074.659922
TTGTAGG2400.074.600661
AGAGGGC9550.074.4830863
AGGGAAT11550.072.8319555
AGTAGGG11200.072.763272
Produced by FastQC (version 0.11.5)