FastQCFastQC Report
Mon 27 Feb 2023
SRR3127806.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127806.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2386232
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195910.8210014784815558No Hit
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG46450.19465835677335647TruSeq Adapter, Index 27 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG44020.18447493789371694TruSeq Adapter, Index 27 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC42780.1792784607699503TruSeq Adapter, Index 27 (100% over 24bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC26130.10950318326130905No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT84900.084.5057451
CGTAGGG6250.082.719672
AGGGATG57900.079.46785
ATAGCGG4500.076.3193051
ATAGGGA37850.075.746063
AAGGGAT73350.075.0971764
TAGCGGG12450.074.746692
AGTAAGG16750.074.431211
AGGGATT55200.073.989095
CGAAGGG23600.073.486992
TAGGGAC14850.073.427324
TAAGGGA36250.072.9955753
GAGGGAT62200.072.766594
AGGGATC32950.072.4594965
TAGGGAT36750.072.386114
ACGGGAT12650.072.078764
GTAGGGA29350.072.0610353
TAGGGCG4900.071.9384844
GGTAAGG13600.071.607011
AAGGGAC31150.071.5181664
Produced by FastQC (version 0.11.5)