FastQCFastQC Report
Mon 27 Feb 2023
SRR3127777.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127777.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1662375
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107690.647808105872622No Hit
GCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTG34480.20741409128505903No Hit
CCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTG30870.185698172794947No Hit
CTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC29220.17577261448229192No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT49500.085.118031
CGTAGGG4050.080.081072
GGGCGAT9000.079.375166
CGAAGGG15950.078.09462
ATAGCGG1700.077.451151
AGGGATT36600.077.048965
TAGGGCG3050.077.046644
AAGGGAT50450.076.94894
CGAGGGA12900.076.875753
AGGGATG42100.076.4724665
GAGGGAT41600.075.8075944
CGGGATT7700.075.0777055
TAGGGAT22950.074.951784
GCGGGAT10850.074.5044564
AGGGAAT28950.074.0308765
AGGGATC24700.074.020035
GGCGATG6250.073.693577
GGCCGAT1350.073.10877
TAACGGG5000.072.386322
AGAGGGA59800.072.228893
Produced by FastQC (version 0.11.5)