FastQCFastQC Report
Mon 27 Feb 2023
SRR3127774.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127774.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1401795
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120200.8574720269368917No Hit
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG36760.2622352055757083No Hit
CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG35360.25224801058642665No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC33790.24104808477701806No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC29750.212227893522234No Hit
AGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT14830.10579292977931866No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT42550.086.017551
CGTAGGG4200.082.798872
ACGGGTA2350.079.989714
GGTAAGG7000.076.6151
AGGGTAT7950.076.254345
AGTACGG1300.075.9946441
AGGGATG26700.074.979125
AAGGGTA10350.074.4638444
TAGCGGG5300.073.594312
AGGGCAT13600.072.909745
AGACGGG6900.072.874682
AGAGGGC19750.072.3353
TAGGGCA9600.070.980454
TAGACGG1550.069.807671
CGAGGGA8350.069.787423
GAGGGAT22300.069.753364
TAAGGGA13150.069.686853
GGATGGC19450.069.579927
AAGGGCA22350.069.176784
AGTAGGG20450.068.939782
Produced by FastQC (version 0.11.5)