FastQCFastQC Report
Mon 27 Feb 2023
SRR3127759.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127759.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1637430
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151980.928161814550851No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG37470.22883420970667448No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG37390.22834563920289724No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC32150.19634427120548667No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC19140.11689049302870962No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT51700.085.243281
TAGGGCA7300.078.54464
AGGGATG27100.075.439595
ATAGCGG3500.073.910431
TAGGGCG4000.072.84694
AAGGGAT34400.072.273094
TAGGGAT14500.071.955564
AGTAGGG26600.071.733792
ATAGGGC11000.071.351513
GTAGGGA14550.070.4162453
CGAAGGG18150.069.914382
ATAGGGA18400.069.730733
TAGCGGG9150.069.341312
ATGCGGG9100.069.205852
AGAGGGC23200.068.876383
AAGGGTA11000.068.787984
GAGGGAT21050.068.766674
TATCGGG1100.068.3607252
AGGGCAT17300.068.187845
ACTAGGG7400.067.9565662
Produced by FastQC (version 0.11.5)