FastQCFastQC Report
Mon 27 Feb 2023
SRR3127757.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127757.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1541683
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149920.9724437514067418No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG33690.2185274145203651No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG32590.2113923549782932No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC27460.17811703184117617No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC17480.11338258254128766No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT51000.085.784071
CGTAGGG3700.081.504412
TAGGGCG3550.079.638994
AGGGATG26400.077.968025
GAGGGAT22600.074.015514
TAGGGCA7500.073.506794
ATAGGGC10400.073.398043
TAGCGGG8850.072.942422
ATAGCGG4600.071.741281
AGTAGGG23800.071.669512
GGTAAGG9200.071.2288361
TAGAGGG24750.071.012772
AAGGGAT29750.070.956784
TAGGGAT15600.070.07554
AGGGAAT18950.069.9336555
CGAAGGG16050.069.5786742
GTAGGGC6400.069.2071153
AGTAAGG9550.069.1120151
ATAGGGA17300.068.909213
AGAGGGA37100.068.456983
Produced by FastQC (version 0.11.5)