FastQCFastQC Report
Mon 27 Feb 2023
SRR3127724.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127724.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2372247
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT522812.2038598847421875No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTT38880.16389524362345068No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCT36840.1552958018283931No Hit
CGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCT27680.11668262200352662No Hit
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC27240.11482784043988674Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG25090.10576470325391917No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24990.10534316198945556No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT149350.088.239671
CGTAGGG6950.075.924432
TAGGGCA17700.073.7318044
TAGGGTA8450.070.810124
TAGAGGG48850.070.40552
ATAGGGC16600.070.386923
ATGCGGG11750.070.1695252
GAATAGG15200.069.150161
AGTAGGG41400.068.736112
ATAGGGA18950.067.3764953
AGGGCAT23200.066.845855
AAGAGGG90800.066.7791752
AGAGGGC39950.066.74953
ATAGAGG19050.066.556311
GGATAGG14200.066.385551
ATAAGGG33950.066.334042
TAAGAGG18850.065.76221
TATAGGG21500.065.521082
GATAGGG34950.065.379692
GTAGGGA16650.065.365123
Produced by FastQC (version 0.11.5)