FastQCFastQC Report
Mon 27 Feb 2023
SRR3127709.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127709.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2260446
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233711.0339110069428776No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26220.11599480810424137No Hit
GCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG24670.10913775423080224No Hit
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG22900.10130744109790722No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT70400.084.855451
ATAGGGA22800.070.848183
GAATAGG14350.070.257871
AGGGCAT28600.069.178625
ATAGGGC17200.068.99883
TAGAGGG43050.068.918662
TAGGGCA17800.068.78964
CGTAGGG5500.068.500972
ATAGAGG18150.068.007651
CGAAGGG25350.067.994312
AAGGGCA46300.067.742734
AAGAGGG85600.067.61572
GTAGGGA19400.066.757553
GGATAGG14700.066.662121
TAGACGG4250.066.51131
TAGGGAT17800.066.4084244
GGTAAGG13650.066.267671
TGTAGGG17600.066.0927352
ATAAGGG34650.065.7826842
AGTAGGG36450.065.764222
Produced by FastQC (version 0.11.5)