FastQCFastQC Report
Mon 27 Feb 2023
SRR3127704.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127704.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3086561
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT399411.2940291800486043No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT118250.086.462011
TAGGGCA24350.075.8471764
AGGGTAC15600.073.2027745
AGGGCAT37900.072.6614765
AGGGATG47200.071.586775
AGTAGGG51700.070.90062
AAGGGCA67050.069.3876654
GAATAGG22200.068.880631
AGTAAGG23050.068.790071
TAAGGGA29300.068.3262943
AAGAGGG120150.068.056772
AGAGGGC49550.068.0019453
CGAAGGG30100.067.915462
TAGAGGG58350.067.6525652
ATAGGGC23750.067.276153
TAAGAGG28650.067.168471
ATAGGGA32250.066.5935753
AGGGCAA44750.066.3697055
GTAAGGG50450.066.323082
GAGGGAT48400.065.8309564
Produced by FastQC (version 0.11.5)