FastQCFastQC Report
Mon 27 Feb 2023
SRR3127698.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127698.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3156151
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT301970.9567666439279997No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT107200.085.400781
TAGGGCA26350.075.4430544
AGGGTAC13900.074.381925
CGTAGGG9550.073.8154752
ATAGGGC24050.072.692123
ATAGCGG7600.071.805591
AGGGCAT41750.071.4787145
AGGGATG55000.071.0064545
AAGGGAT69450.070.510694
TAGAGGG59950.070.395792
AGTAGGG56150.070.054372
TAGCGGG18850.069.808162
AGAGGGC56050.069.676273
AAGGGCA64800.068.970764
GTAGGGC20950.068.6431053
AGGGATT47700.068.178475
TAGGGAT29100.068.1520544
GAGGGAT52350.067.867934
AGGGATC35100.066.811765
TATAGGG29900.066.6429442
Produced by FastQC (version 0.11.5)