FastQCFastQC Report
Mon 27 Feb 2023
SRR3127695.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127695.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2549580
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1078304.229324045529068No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCT87390.34276233732614786No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTT83990.3294268075526165No Hit
CGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT56210.22046768487358703No Hit
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG49200.19297296025227684TruSeq Adapter, Index 22 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTC42440.16645878929078514No Hit
CGTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTC31920.1251970912856235No Hit
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC31840.12488331411448161TruSeq Adapter, Index 20 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT300400.090.381691
ATAGGGC18050.075.766353
TAGGGCA16200.073.685044
ATAGCGG5950.069.5816961
AGATAGG15450.068.8191451
TAGAGGG45500.068.4798362
AGTAGGG37550.068.3350142
GTAGGGC12900.068.125943
GAATAGG16600.068.019351
AAGGGCA43500.067.522944
GTTTTTT403750.067.162042
GGTAAGG14600.067.025461
CGTTTCT13750.066.7207951
ATGAGGG37300.066.147142
AAGAGGG88600.065.879232
TAGCGGG14850.065.8260042
AGGGTAG11200.065.458675
AGAGGGC41000.065.106613
AGGGATG32850.064.950295
AGGGCAT27450.064.886975
Produced by FastQC (version 0.11.5)