FastQCFastQC Report
Mon 27 Feb 2023
SRR3127691.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127691.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2387425
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT331681.3892792443741688No Hit
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG25790.10802433584301077No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT111650.087.913471
AGGGATG49500.077.4702455
ATAGGGC18150.077.086273
TAGGGCA17300.075.9723054
CGTAGGG6500.075.373242
AAGGGAT60250.074.825924
TAGGGAT25450.074.410714
ATAGGGA31450.074.294763
TAGGGCG5300.073.773344
AAGGGCG12350.073.618614
CGAAGGG25100.073.3838042
CGAGGGA15700.072.912813
TAAGGGA29000.071.961953
GTAGGGA22900.071.588123
GAATAGG14900.071.473831
AGGGCAT25450.071.0923545
AGGGATT40150.071.04825
GAGGGAT41500.070.94624
ATAGCGG4650.070.936531
ATGCGGG10900.070.878512
Produced by FastQC (version 0.11.5)