FastQCFastQC Report
Mon 27 Feb 2023
SRR3127690.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127690.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2971676
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268020.9019152828235649No Hit
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG44300.14907412517380764Illumina PCR Primer Index 7 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG42240.14214201009800528Illumina PCR Primer Index 7 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC41930.14109882773222923TruSeq Adapter, Index 23 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT94650.084.922121
ATAGCGG6300.078.387741
CGTAGGG7900.075.5562
ATAGGGA35700.074.908163
AGGGATG65700.074.7535
CGAAGGG30050.074.44832
TAGGGCA23100.073.852484
AGGGCAT36100.073.816525
AAGGGAT75800.072.975594
TAGCGGG15700.072.444942
AAGGGCA59600.072.309194
TAGGGAT33550.071.8613364
AGATAGG19600.070.7889251
TAAGGGA34150.070.738763
ATAGGGC24800.070.687433
TAGGGCG5550.070.283854
GGTAAGG19550.069.76711
GCGGGAT19000.069.258774
AGGGATC30750.069.082985
GAATAGG22050.068.4692841
Produced by FastQC (version 0.11.5)