FastQCFastQC Report
Mon 27 Feb 2023
SRR3127683.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127683.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3518254
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT580971.6513020378858378No Hit
CTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC37770.1073543865792521No Hit
CCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG36030.10240875161372659No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT172950.087.921311
AGGGTAC29750.075.5096745
AAGGGTA39550.074.8610154
ACGGGTA4850.073.643354
CGTAGGG11100.073.246152
TAGGGCA27850.072.730034
TAGGGTA15600.072.602924
TAGCGGG18800.070.4941252
TAGAGGG65600.070.3508452
GGTAAGG21950.070.090091
ATAGGGA31400.069.8954543
AGTAGGG60750.069.778552
ATAGCGG8100.069.7010961
AGGGTAT26950.069.578615
GAGGGTA31700.069.530484
GAATAGG23600.068.977511
AGGGTAG21250.068.5595
AGGGTAA25350.068.40855
ATAAGGG53700.068.262472
AGGGCAT38150.067.876395
Produced by FastQC (version 0.11.5)