FastQCFastQC Report
Mon 27 Feb 2023
SRR3127679.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127679.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1303963
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126690.9715766474968999No Hit
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG41390.3174169819235669No Hit
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG36230.2778453069603969No Hit
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC33050.25345811192495493TruSeq Adapter, Index 13 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT16070.12323969315080259No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC16040.12300962527310975No Hit
AAAGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTT13760.10552446656845325No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA13170.10099979830716056No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT42450.084.693791
CGTAGGG3550.080.9293062
TACGGGT1050.080.7397843
TAGGGCA8250.077.0697864
ACGGGTA1450.074.70754
AAGGGCA21350.074.5629654
AGGGCAT10650.074.133385
AGGGATG23900.072.754185
AGTACGG1750.072.6993261
ATAGCGG1800.070.67991
TAGAGGG19900.070.29232
CGGGAAT2750.070.065675
AGGGCAA16100.069.179375
TAAGGGA12200.069.10313
AGGGAAT14950.068.842575
AGTAGGG21750.068.211192
AGGGTAC5900.067.705045
TAGCGGG4250.067.599782
ATAGGGA14250.067.42483
CGAAGGG10650.067.219982
Produced by FastQC (version 0.11.5)