FastQCFastQC Report
Mon 27 Feb 2023
SRR3127678.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127678.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2248052
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230341.0246204269296262No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26420.11752397186541948No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT70550.084.0094451
ATAGGGC16100.072.977983
TAGGGCA14700.071.6141364
ATAGGGA18950.071.4267653
ACGGGTA3300.071.207234
CGTAGGG5050.070.7309652
AGTAGGG37800.070.000952
TAGGGAC10750.068.1999744
AGTAAGG12850.067.354921
GCATAGG7350.067.198041
AAGGGCA46300.067.094754
ACTAGGG10250.066.9447252
TATAGGG18450.066.740942
AGGGCAT23350.066.62075
AAGAGGG87100.066.316432
ATAAGGG32050.066.282332
ATAGAGG17000.065.854071
CGAAGGG24400.065.682842
GTAGGGA15200.065.549593
GAGGGAT22950.065.5292744
Produced by FastQC (version 0.11.5)