FastQCFastQC Report
Mon 27 Feb 2023
SRR3127667.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127667.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1908642
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192191.0069463000395045No Hit
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG35230.18458149825897158No Hit
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG33090.1733693379900474No Hit
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC32810.17190232636607597TruSeq Adapter, Index 13 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24220.12689650547352516No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT58350.083.212481
CGTAGGG3900.074.899622
AGTAGGG30600.073.1348952
TAGACGG2650.072.9340061
AGTAAGG13100.071.969881
AGACGGG10950.071.4243852
TAGGGCA13300.069.7858054
TAGGGTA6100.069.512864
GGTAAGG10050.069.420511
TAGAGGG30950.068.958862
ATAAGGG25050.068.6495362
ATAGGGA16800.068.427873
AGAGGGC29300.068.339813
ATAACGG1400.067.3432541
ATAGAGG11350.067.283911
TAACGGG5900.066.27942
ATAGGGC11100.066.214653
ACGGGTA2350.066.160474
TATAGGG17750.065.827262
GAATAGG10200.065.6266561
Produced by FastQC (version 0.11.5)