FastQCFastQC Report
Mon 27 Feb 2023
SRR3127665.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127665.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3229708
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT338211.0471844513497814No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC45600.1411892344447238No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG32750.10140235587861193No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT112700.084.907531
CGTAGGG9400.078.994652
ACGGGTA4250.075.194914
AGGGATG66650.074.320555
ATAGCGG4750.073.292121
AGGGTAC18400.072.283155
TAGGGTA15350.071.337194
TAGGGCA23900.070.9868244
AAGGGAT71600.070.560874
ATAGGGC23900.070.3969043
AGTAGGG58300.070.213092
CGAAGGG31800.070.051862
CGAGGGA19750.069.48393
TAGAGGG62650.069.4637762
GAGGGAT51900.069.2727664
TAACGGG9650.069.1559452
AGGGTAA22900.068.956035
AGGGCAT36300.068.747415
AAGAGGG115850.068.6798552
GAGGGTA27300.068.343364
Produced by FastQC (version 0.11.5)