FastQCFastQC Report
Mon 27 Feb 2023
SRR3127661.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127661.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2109453
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189800.899759321492349No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC43960.2083952569694608No Hit
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG40620.19256176838260913No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG38790.18388653361795687No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT73450.086.810991
ATAGCGG3600.081.1629641
CGTAGGG4150.080.586492
TAGGGCG5650.079.2003564
AAGGGAT58450.078.492194
AAGGGCG11600.076.339944
ATAGGGA28300.076.064453
AGGGATT42000.075.8634645
AGGGATG41800.075.664315
TAAGGGA26750.075.365393
TAGGGCA17100.075.200124
CGAAGGG18400.075.0069962
ATAGGGC16000.074.7765963
AGGGCAT24400.073.381655
ACGGGAT8350.073.334594
AGGGATC26300.073.2622765
CGAGGGA15350.073.033233
CGAGGGC8750.072.1354453
AGTACGG1900.071.9305041
GAGGGAT45000.071.70184
Produced by FastQC (version 0.11.5)