FastQCFastQC Report
Mon 27 Feb 2023
SRR3127651.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127651.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1640833
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118470.7220113198600955No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG30650.18679536552470605No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC30410.18533269382076056No Hit
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG28320.17259526106556852No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC18260.11128493880851982No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT49950.086.144551
CGTAGGG5850.081.143952
TAGGGCA13300.079.1541754
CGAAGGG14950.076.079012
ACGGGTA2250.075.1964654
AATGCGG4650.074.83781
GCGGGAT10150.074.548224
AGGGATG35700.074.51195
GCGTAGG3350.074.4000551
AAGGGCG6950.073.708764
AGGGATT32000.073.580925
TAAGGGA20850.073.483353
AAGGGAT45950.073.13044
TAGGGTA4700.072.9965744
GAGGGAT37450.072.7869644
AAGGGCA31950.072.666384
ATAGGGA22550.072.528753
TAGGGAT20550.072.49784
TAGCGGG8300.072.480732
ATGCGGG9000.072.0654752
Produced by FastQC (version 0.11.5)