FastQCFastQC Report
Mon 27 Feb 2023
SRR3127648.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127648.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2527688
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229360.9073904690768797No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC59490.23535341387069925No Hit
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG42210.1669905463016005TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC40240.159196862903966No Hit
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG36630.14491503698241237TruSeq Adapter, Index 22 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31680.125331923876681No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT71550.084.069961
CGTAGGG6450.074.322382
ATAGGGC15700.073.638683
CTACGAC14450.073.502575
TACGACC14550.072.6715246
GTACGGG5700.070.9092252
AGTAGGG43400.070.821832
TCCGCTA15100.070.701541
GAATAGG16650.070.616421
TAGGGCA16250.070.5664754
AGGGATG39700.070.553365
GGTAAGG14200.070.214611
AGGGCAT24750.069.6871645
TAGGGTA8350.069.2278064
GTAGGGA22100.068.687993
CGAAGGG25050.067.5422
TAGCGGG14650.067.36912
AGGGTAA11300.066.959065
AGAGGGC36200.066.7306063
AAGAGGG86700.066.6211242
Produced by FastQC (version 0.11.5)