FastQCFastQC Report
Mon 27 Feb 2023
SRR3127646.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127646.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2361311
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223270.9455340698450988No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC53710.22745839069906507No Hit
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG37550.1590218315164754TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC35060.14847684188995014No Hit
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG31930.13522149348391635TruSeq Adapter, Index 22 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30920.1309442085350045No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT70750.084.084631
CGTAGGG6850.079.7781142
ATAGGGC16250.076.53623
TAGGGCA16300.074.278284
ATAACGG2500.073.5374151
CTACGAC11150.072.915445
AGTAGGG38750.071.72932
ACGGGTA3550.070.3337554
ATAGGGA19700.070.306813
AGGGATG33800.069.93585
TACGACC11700.069.4877856
GTAGGGA21150.069.050633
GCTACGA11800.068.669324
CGAAGGG23050.068.059642
AAGGGAT36250.067.059144
CGCTACG12050.066.853693
AAGAGGG82000.066.816262
TAGCGGG11650.066.318432
GAGGGAT28200.065.820844
CGAGGGA12350.065.6111763
Produced by FastQC (version 0.11.5)