FastQCFastQC Report
Mon 27 Feb 2023
SRR3127636.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127636.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1166837
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105780.9065533574955199No Hit
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG29670.2542771612487434No Hit
CCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG27530.23593698177208985No Hit
CTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC27030.23165189310932036No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC24410.20919802851640806No Hit
AGCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCT11850.10155660130763766No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT39200.086.164251
CGGGTAT1000.079.893125
TAGCGGG6050.079.236242
AGTACGG2350.078.070241
AGGGATG26400.077.970555
AAGGGAT27050.074.533324
CGAAGGG12200.074.3492362
AAGCGGG12450.073.988762
CGTAGGG3200.073.434322
GGATGGC20550.072.4887547
ATAGCGG2600.072.3727951
TAAGCGG2500.071.5043261
TAGAGGG18600.070.7496262
CGGGATG5950.070.296465
AGAGGGA26100.070.2268453
AGTAGGG17400.069.956732
TAGGGCA6450.069.947464
GAGGGAT18750.069.929984
AGGGAAT16700.069.790395
TAGGGAT9850.069.658974
Produced by FastQC (version 0.11.5)