FastQCFastQC Report
Mon 27 Feb 2023
SRR3127596.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127596.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1359642
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172521.2688634214006334No Hit
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG36790.27058593365018146No Hit
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC35780.26315750763804Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG35500.2610981420109117No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT21110.15526145853099566No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT50500.085.411051
TAGGGCG2550.079.249574
ATAGGGC8800.075.301763
GAATAGG6200.075.1068651
AGTAGGG21900.073.821522
AATAGGG17850.073.193952
TAGAGGG22050.072.04052
GTAGAGG9600.071.04491
TAGTAGG5500.070.9825441
AGGGTAC6100.070.880485
ATAAGGG16100.070.641212
GTACGGG3750.070.1819462
TAGGGCA8750.069.823874
TGAAGGG23250.068.9286962
AGTAAGG9050.068.086161
AGGGTAA5600.067.977575
TATAGGG10500.067.585922
TAGCGGG5650.067.3762
AAGGGTA8950.067.2133564
TAGGGGT7850.067.052814
Produced by FastQC (version 0.11.5)