FastQCFastQC Report
Mon 27 Feb 2023
SRR3127581.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127581.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3104340
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT332541.0712099834425353No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC46630.1502090621516973No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT100950.084.876531
TAGGGCA22250.074.562044
ATAGGGC23850.073.895153
AAGGGTA24500.071.742844
AGTAGGG54250.070.950792
AGGGATG52550.070.741925
ATAGCGG6800.070.5789641
CGTAGGG7800.070.495982
CGAAGGG30950.070.0024642
GTACGGG8100.069.045442
TAGCGGG16250.068.5437852
TAGAGGG59350.068.3381352
GAATAGG18000.068.226331
AGGGCAT33450.068.14265
ATAGGGA29200.068.081733
TAGGGCG6200.067.463894
AGATAGG19200.067.148041
AGGGTAC17850.067.139025
AGAGGGC51750.066.204923
AAGAGGG115050.066.13532
Produced by FastQC (version 0.11.5)