FastQCFastQC Report
Mon 27 Feb 2023
SRR3127566.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127566.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1698029
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168140.9902068810367785No Hit
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG41500.24440100846334192Illumina PCR Primer Index 11 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG41330.2433998477057812RNA PCR Primer, Index 41 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC38420.22626233120871317RNA PCR Primer, Index 41 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT60800.085.8380661
CGTAGGG4350.079.950022
AGGGATT37750.076.5657045
TAGGGCA12950.076.2123954
AGGGCAT21650.075.9777155
ATAGGGA25350.075.455783
GGTAAGG12350.075.4484251
AGGGATG31400.075.435715
TAAGGGA25250.075.01013
AAGGGAT46350.074.9325944
TAGCGGG6150.074.890562
GAGGGAT35850.074.46214
CGAAGGG14300.073.947352
AGTAAGG11350.073.803351
AGTACGG2450.072.991031
GTAGGGA20150.072.7705463
AGAGGGC28750.072.417243
AGAGGGA55400.071.938643
AGGGAAT24300.071.75365
AAGGGCA34650.071.479824
Produced by FastQC (version 0.11.5)