FastQCFastQC Report
Mon 27 Feb 2023
SRR3127556.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127556.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1787248
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171980.9622615328146961No Hit
GCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG20590.11520505268435048Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC20190.11296697492457679Illumina Single End Adapter 1 (95% over 22bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC19690.11016937772485969No Hit
CCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG19110.10692416497318782Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT54500.085.017521
CGTAGGG5300.079.9971162
TAGGGCG3700.073.84724
TAGGGCA10150.073.797014
TAGGGTA6850.072.899254
AAGGGCA29100.072.525844
AGTAGGG29950.072.040442
GAATAGG11750.071.806961
ATAGGGC12700.070.84963
CGAAGGG17550.070.328592
TAGAGGG34650.069.882372
AGGGCAT16400.069.632485
AGGGTAC8650.069.5413745
AGGGATG23750.067.870325
AGTAAGG10300.067.729191
AAGGGTA14000.067.635664
ATAGCGG2950.067.108671
ATGAGGG29950.066.8497542
ACGGGTA2400.066.738344
AAGGGAC16950.066.425674
Produced by FastQC (version 0.11.5)