FastQCFastQC Report
Mon 27 Feb 2023
SRR3127541.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127541.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2764964
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264360.956106480952374No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC47240.17085213406033498No Hit
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG42880.1550833934908375No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG38030.13754247794907998No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT89500.083.829791
CGTAGGG6600.082.082622
TAGGGCG6250.076.8807144
TAGGGCA18900.076.02134
CGAGGGA18500.074.100053
AAGGGAT65900.074.057944
ATAGCGG5350.073.9952551
GTACGGG6400.073.606692
AGGGATG48600.073.586795
GAGGGAT51950.073.450834
GTAGGGC14300.073.133143
ATAGGGC20650.072.544483
AGGGATC33000.072.48625
CGAAGGG25850.072.348122
TAAGGGA34000.072.0479663
ATAACGG2700.071.564641
TAACGGG8600.071.21022
AGGGATT50900.071.0002755
ATAGGGA33550.070.34653
AGAGGGA80950.069.833153
Produced by FastQC (version 0.11.5)