FastQCFastQC Report
Mon 27 Feb 2023
SRR3127491.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127491.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2846576
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311971.0959482550263897No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC44950.15790901068511784No Hit
GCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG42800.15035607691486194No Hit
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG39670.13936041054234982No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC34110.1198281725132229No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT98450.085.717211
CGTAGGG8600.076.004272
TAGGGCA16000.073.139034
AGTAGGG43550.069.5375442
TATAGGG21750.069.401272
AGGGCAT22650.068.887395
GAATAGG19300.068.037811
ATAGGGA26050.067.83443
ACGGGTA3050.067.798914
ATAGGGC16600.067.6643143
AGGGATG38800.067.46735
TAGAGGG47800.067.093142
TAGGGTA9150.066.771654
AGGGTAC12950.066.4127045
AGAGGGC36250.066.249533
GTAGGGC15300.066.04153
CGAAGGG30050.066.037352
AAGGGTA19300.065.990574
GGTAAGG17200.065.946551
ATAGAGG17750.065.759241
Produced by FastQC (version 0.11.5)