FastQCFastQC Report
Mon 27 Feb 2023
SRR3127490.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127490.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3410249
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT398561.1687123139688627No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC58410.17127781578412604No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT36230.10623857671389979No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT128100.085.1538241
CGTAGGG9300.079.3502352
TAGAGGG64850.072.625532
AGGGATG55950.071.4925845
AGTAGGG53400.069.449212
ATAGGGC17950.068.868853
ATAGCGG9300.068.757681
ATAGAGG23900.068.461531
GAATAGG25050.068.134051
ACGGGAT11600.068.074244
GTACGGG7950.067.992692
CTACGAC11250.067.685255
AAGGGTA25100.067.415584
GGTAAGG21300.066.884931
AGGGCAT29550.066.8072055
TAGGGCA20950.066.1865164
ATAAGGG51300.065.420282
CGGGATG13900.064.9258965
GTAAAGG28650.064.496061
GAGGGAT53300.064.464654
Produced by FastQC (version 0.11.5)