FastQCFastQC Report
Mon 27 Feb 2023
SRR3127488.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127488.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2991167
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT324191.0838244738591996No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC51250.17133780895550133No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT33340.11146151318197882No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG30150.10079677931723638No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT102250.084.968761
CGTAGGG9000.079.429082
AGTAGGG45000.073.4718862
AAGGGTA19600.071.933524
ATAGCGG7200.071.903671
AGTAAGG16500.070.738951
CTACGAC11300.069.45515
AGGGATG47350.069.378245
GAATAGG21000.068.803411
TAGAGGG57950.068.090562
TAGGGTC7050.067.9950264
AGGGAAT31700.067.7521445
GTAGGGA23850.067.194253
ATAGGGC14950.066.32963
AGACGGG20500.066.071932
TAAGGGA26600.065.724523
AGGGTAC12950.065.686325
ATAACGG2750.065.0341951
TAGGGCA15550.064.979174
GGTAAGG20900.064.854051
Produced by FastQC (version 0.11.5)