FastQCFastQC Report
Mon 27 Feb 2023
SRR3127487.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127487.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3177083
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT419271.3196696466538644No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC35150.11063607718149006No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT134450.085.757821
ACGGGTA3550.072.824494
GGTAAGG19000.071.525081
AGGGATG42800.070.397535
TAGAGGG54900.069.522732
ATAGGGA28000.068.660733
GAATAGG22900.067.968331
ATGTAGG11150.067.899281
ATAGCGG8750.067.17631
CGTAGGG8750.067.1498642
TGTAGGG23400.066.8915942
TAGGGCA18650.066.537774
GTACGGG7300.066.321982
AGGGCAT23950.065.7479865
TAAGGGA27100.065.737483
AAGAGGG117500.065.366812
AAGGGCA45750.065.2417764
GTAGGGA24000.065.219313
AGTAGGG58100.064.7227552
TAAGAGG25500.064.541931
Produced by FastQC (version 0.11.5)