FastQCFastQC Report
Mon 27 Feb 2023
SRR3127485.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127485.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2763907
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT332711.2037669863711045No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC30310.10966360300835015No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT108600.086.058151
ATAACGG2700.074.94581
TAGAGGG46650.071.160962
TAGGGCA12950.071.130364
TAGGGTC7700.069.579744
GGTAAGG18250.069.363661
AGGGATG38100.069.200085
CGTAGGG7900.069.0432
AAGAGGG98500.068.311232
ATAGGGC14900.068.129653
AGGGCAT22800.066.3727345
AGTAGGG49850.066.215652
ATAGGGA24350.066.200893
GAATAGG18800.066.082841
GTACGGG6850.065.897572
GTAGGGA20000.065.325613
TAAGGGA21250.065.0215153
TGTAGGG21850.064.9896552
AGAGGGC37600.064.870643
TAAGAGG20450.064.4328461
Produced by FastQC (version 0.11.5)