FastQCFastQC Report
Mon 27 Feb 2023
SRR3127484.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127484.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3476812
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT489351.4074675305998714No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT156150.086.728651
ATAGGGC20000.074.0380863
AGTAGGG56950.070.326742
TAGGGCA21500.069.0912864
AGAGGGC57250.068.2338943
CGTAGGG8550.068.175792
AGGGCAT35650.067.908215
TAGAGGG66450.067.8419342
GAGGGAT46500.067.631314
AGGGATG49250.066.622945
AAGAGGG138750.065.828572
ATAGGGA33100.065.044723
GGTAAGG20800.064.882961
GAATAGG25200.064.750181
AATAGGG54750.064.05152
TAGGGTA12550.064.051164
GATAGGG54250.064.035282
AGGGAAT36450.063.7094765
TAGGGCG6500.063.6420174
AAGGGTA23650.063.6053164
Produced by FastQC (version 0.11.5)