FastQCFastQC Report
Mon 27 Feb 2023
SRR3127483.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127483.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3017813
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT393191.3028971642709473No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT124200.087.710581
ATAGGGC18500.072.859943
TAGCGGG18150.070.1102752
TAGGGCA15800.070.0979464
AGAGGGC47600.069.70443
TAGGGTA10700.069.593364
TAGAGGG56200.068.933452
ATAGGGA25950.068.651333
AGTAGGG50100.068.389782
GTAGGGA22200.068.359263
CGTAGGG8900.067.782012
GGTAAGG17750.067.217721
GAGGGAT38850.066.842874
AAGAGGG116500.066.790652
TAAGGGA26000.066.706623
GATAGGG46600.066.547922
ATAGCGG7100.066.420671
AGGGATG47600.065.4556665
AGAGGGA69350.065.104883
AGGGAAT31200.064.164635
Produced by FastQC (version 0.11.5)