FastQCFastQC Report
Mon 27 Feb 2023
SRR3127481.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127481.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2319194
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286881.2369814685619227No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28880.12452602067787343No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT95900.086.395911
CGTAGGG7050.075.34182
GGTAAGG12700.075.161981
ATAGGGC16100.071.821743
ACGGGAT6750.068.941084
AGTAGGG38450.068.826982
TAGGGTA7150.068.371324
TAGAGGG39700.067.96232
TAGGGCA15600.067.796084
AAGAGGG82900.066.96412
CGAAGGG23950.066.92612
AGGGATG37050.066.860425
GTAGGGA20600.066.172493
ATAGGGA22350.066.038743
AAGGGGC68350.065.883054
GAATAGG13450.065.726651
TAAGGGA20100.065.479993
AGTAAGG15900.065.062611
ACGGGTA2250.064.762834
GAGGGAT29200.064.551774
Produced by FastQC (version 0.11.5)