FastQCFastQC Report
Mon 27 Feb 2023
SRR3127478.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127478.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2671810
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313451.1731747392217262No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC30800.11527765821671451No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT100250.085.355831
TAGGGCA16250.074.631664
AGTAGGG45000.073.8522342
AGGGCAT25500.072.6294565
CGTAGGG8000.072.27232
ATAGGGA23400.071.513973
AGTAAGG16500.070.381611
ATAGGGC16750.070.158763
TATAGGG19950.069.272552
AGGGATG43400.068.559995
TAGCGGG14250.067.2933352
TAGGGAT20300.067.151964
TAGACGG3650.066.981771
TAAGGGA22500.066.435683
GATAGGG39150.066.157122
TAACGGG10050.065.949242
CGAAGGG29300.065.937262
AAGGGAC23600.065.729254
ATGCGGG17600.065.7020952
ATGAGGG46550.064.8294142
Produced by FastQC (version 0.11.5)