FastQCFastQC Report
Mon 27 Feb 2023
SRR3127476.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127476.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2336481
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248191.0622384688769135No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC24860.10639932445416847No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24750.10592853098313232No Hit
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG23380.10006501229840946No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT77500.084.893111
TAGGGCA12900.074.685434
AGTAGGG42100.073.159832
ATAGGGA19000.071.237823
AGTAAGG15600.070.5924451
TAGACGG3150.070.218941
ATAGAGG16450.069.80571
TATAGGG15850.069.422472
GTAGGGA20000.068.615883
AGGGATG37700.068.438865
CGTAGGG7050.068.033772
ATAGGGC14600.067.920583
GAATAGG13450.067.8807141
TAGGGAT16500.067.220214
TAGAGGG41650.067.17622
AGGGCAT21800.066.6152655
CGAAGGG23700.066.269182
TAGTAGG12500.066.262771
GTAAGGG34200.065.3101962
TAGGGCG4250.065.243134
Produced by FastQC (version 0.11.5)