FastQCFastQC Report
Mon 27 Feb 2023
SRR3127472.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127472.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2572100
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT312541.2151160530305976No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC73090.28416469033085806No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT100450.085.5666961
CGTAGGG7250.074.5638962
AGGGATG53100.074.452395
TAGGGCA15450.073.023044
TAAGGGA22950.071.0747763
TAGAGGG50450.071.000682
ATAGCGG5450.070.745021
GGTAAGG15500.070.681011
AGGGCAT25900.070.603625
TAGACGG3650.069.563181
AGAGGGC39150.069.400853
CTACGAC13400.069.1096345
GGATGGC45800.068.9759757
AAGGGCA42100.068.223864
ATAGGGC16250.067.980323
ATAAGGG38000.067.790012
GAGGGAT39050.067.774224
AGAGGGA70850.067.608773
AGTAGGG42700.067.373992
ATAGGGA29650.067.063223
Produced by FastQC (version 0.11.5)