FastQCFastQC Report
Mon 27 Feb 2023
SRR3127463.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127463.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2433120
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT314941.294387453146577No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC57180.23500690471493393No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28780.11828434273689749No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT95550.085.776671
TAAGGGA23150.072.286743
AGGGATG46500.071.773665
GGTAAGG13450.071.669461
CGTAGGG7350.071.629252
AGGGCAT22750.071.2848745
CTACGAC10650.070.620385
TAGAGGG39250.068.743542
AGTAGGG38150.068.507792
AGAGGGC34000.068.436213
ACGGGTA2750.068.373374
AAGAGGG81700.068.179842
TAGGGCA12450.067.961484
ATAGAGG14400.067.920891
ATGCGGG12800.067.204882
AGGGAAT28300.067.1049655
GGATGGC40300.067.06917
ACGGGAT9700.066.8754964
GTACGGG4150.066.828792
ATAGGGA26250.066.7942663
Produced by FastQC (version 0.11.5)