FastQCFastQC Report
Mon 27 Feb 2023
SRR3127458.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127458.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2475571
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT287701.1621561247889882No Hit
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG43800.17692887822647785No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC40880.165133619678046No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG36520.14752152129751075No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT83650.085.587991
ATAGGGC12000.077.1493453
CGTAGGG6500.075.964872
TATAGGG21700.073.030962
TAGGGTA6050.072.2395254
AGGGATG28000.071.666665
GGAGCTA54350.071.507779
ATAGCGG5100.071.067351
TAGAGGG39450.070.210992
TAGGGCA12600.070.118824
AGTAGGG39850.069.7422562
AGGGCAT19700.069.418315
TAGGGAT12500.069.175944
GTAGGGA17250.068.108013
CGAAGGG25450.067.813342
TAGTAGG8850.067.5476151
GTAGGGC13500.067.184773
GATAGGG38100.067.021122
TAGACGG2950.067.015751
ATAGGGT10600.066.945033
Produced by FastQC (version 0.11.5)