FastQCFastQC Report
Mon 27 Feb 2023
SRR3127457.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127457.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2977003
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT317681.0671134694859226No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC49480.16620742404357672No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT109200.084.7388841
TAGACGG4000.076.407081
ACGGGTA3000.073.638284
AGGGATG52700.072.9574665
CGTAGGG7800.072.915152
TAGGGCA15700.068.558754
TAGAGGG52050.068.179412
ATAGGGA35350.068.0781253
AGGGCAT27800.067.968595
ATAGCGG6950.067.654311
GAGGGAT47900.067.511844
AGAGGGC46950.067.2760853
AGTAGGG47000.067.00452
AAGAGGG114200.066.6358262
AGATAGG19000.066.570061
ATAGGGC17700.066.388643
AGGGCTT31500.066.252075
TAAGGGA33250.066.0164643
TACGGGT2500.065.804433
GGTAAGG18700.065.3750461
Produced by FastQC (version 0.11.5)