FastQCFastQC Report
Mon 27 Feb 2023
SRR3127451.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127451.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1120303
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163741.4615688791335915No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC116281.037933487636827No Hit
CTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC29100.2597511566067394No Hit
CCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG27840.24850419931036513No Hit
GCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG23270.2077116637195473No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA15140.13514201068817988No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCCTA14580.13014336300090243No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGG450.094.040231
CGGGTAT1500.090.893385
GTACGGG2200.087.616662
CGTTTTT63500.087.153821
AGGGATG34200.085.3671955
CGCTACG18150.083.6664663
ATAGCGG2050.082.5719151
TCCGCTA18800.082.0350951
CGTAGGG2650.081.608892
CGACCAA18450.081.2867668
ACGACCA18400.081.2521447
GCTACGA18700.081.205694
GGATGGC30100.081.06377
AGGACGG2450.080.605921
CTACGAC19200.079.3358155
CGAAGGG8050.077.6752
GAGGGAT26200.077.518974
AAGGGTA8450.076.779974
AGGGCTA6800.076.743155
GGAATGC16700.076.57347
Produced by FastQC (version 0.11.5)