FastQCFastQC Report
Mon 27 Feb 2023
SRR3127449.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127449.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1029259
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126671.2306912060035424No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC97020.942619884790903No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCTAGGAAT39550.38425702374232334No Hit
CTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC35490.3448111699776247No Hit
CCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG34390.33412386969654867No Hit
GCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG33720.3276143322526206No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCCTA19220.18673628309298243No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA16020.15564595500257955No Hit
TAGACCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCT13550.13164810800779977No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA12150.11804608946824852No Hit
CGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCT11130.10813604738943258No Hit
AAAGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTT11100.10784457556358507No Hit
AGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCT10960.10648437370962993No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC10380.10084925174324441No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATGG201.8241079E-594.141731
CGGGTAT1550.090.963285
CGTTTTT53000.087.836011
AGGGATG31250.087.0773245
ACGGGTA1800.086.162444
CGCTACG16750.083.613813
GGATGGC27400.083.360877
CGTAGGG2550.083.025842
GCTACGA16900.082.5935754
ACGACCA16650.082.4223867
TAGGGCA5550.082.1401144
AGGGTAC4050.081.230585
CTACGAC17250.080.917765
TCCGCTA17700.080.578931
CGACCAA17100.080.528218
GCGGGAT5200.080.4383544
GACGGGT1700.080.172543
TAGCGGG4200.079.533482
GGAATGC14400.078.982247
GTACGGG1550.078.919192
Produced by FastQC (version 0.11.5)