FastQCFastQC Report
Mon 27 Feb 2023
SRR3127448.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127448.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2328377
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291801.2532334755067587No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC48300.2074406335400152No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24500.10522350976667437No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT84950.085.510211
TAGGGCA16000.073.446364
CGTAGGG6750.073.119932
AGGGATG42750.072.4602055
ATAGGGC14650.071.8721543
AGTAGGG40650.071.4624942
TATAGGG19350.070.933622
ATAGCGG5750.070.320751
AGTAAGG13600.070.179451
TAGCGGG14650.069.626152
CGAAGGG25350.068.607882
GTAGGGT10500.068.493983
TAGGGTA8750.068.225374
TAGAGGG39950.067.890542
CTACGAC8800.067.837735
AGAGGGC35500.067.79413
AGGGAAT23900.067.656695
AGGGCAT20650.067.3785865
GGTAAGG14500.066.4721
ATGCGGG11750.066.408012
Produced by FastQC (version 0.11.5)