FastQCFastQC Report
Mon 27 Feb 2023
SRR3127447.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127447.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2025792
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237921.1744542381448837No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC41490.20480878589707135No Hit
GCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG28420.14029080971787825RNA PCR Primer, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG23010.1135852051938205RNA PCR Primer, Index 16 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC22060.10889568129403217RNA PCR Primer, Index 16 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT20960.10346570625217198No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT69950.085.692461
AGGGATG38200.072.458675
CGTAGGG4950.072.3678742
CTACGAC8700.071.8407445
TACGACC8700.070.760436
TACGGGT2000.070.701513
TAGCGGG11350.070.5976942
GTAGGGT8850.070.302063
GGTAAGG11200.069.4785461
ATAGCGG4350.069.386581
AGTAGGG35000.069.354812
ATAGGGC12450.069.28183
AGTAAGG12050.069.274111
GTAGGGA16650.069.0737463
ATAAGGG28650.068.439392
GAGGGAT27500.068.216244
AGAGGGC28200.068.194373
CGAAGGG20850.067.81922
AGGGTAT8250.067.784675
ACGGGTA1600.067.7556154
Produced by FastQC (version 0.11.5)