FastQCFastQC Report
Mon 27 Feb 2023
SRR3127442.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127442.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677393
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106511.5723516481569784No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC52810.7796065208822648No Hit
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG22420.33097478125696606TruSeq Adapter, Index 14 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC20420.3014498230716881Illumina PCR Primer Index 3 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG16530.24402377940132242TruSeq Adapter, Index 14 (95% over 24bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA11350.1675541377014525No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCGT156.8974256E-494.0121847
TAGCGGG2500.086.49122
CGTTTTT47600.086.341381
CGCTACG9450.085.058653
ACGGGTA950.084.1161654
TCCGCTA9800.083.4906161
GCTACGA9600.083.239954
AGTACGG850.082.982571
ACGACCA9600.080.791727
ACGGGAT3850.080.581874
CGGGATG3450.080.387225
GGATGGC13600.080.186877
CGACCAA9750.080.030888
GATGGCA12550.079.779268
TACGGGT650.079.548773
AGGGTAC3550.079.4469155
AGTAAGG2850.079.1973951
CTACGAC10100.079.119175
TAAGCGG1250.078.9994051
CCGCTAC10400.077.740842
Produced by FastQC (version 0.11.5)