FastQCFastQC Report
Mon 27 Feb 2023
SRR3127441.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127441.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences616169
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86241.3996160144375975No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC44280.7186340111235716No Hit
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG28430.46139938880404563TruSeq Adapter, Index 14 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC26220.42553260550271105Illumina PCR Primer Index 3 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG24940.40475908395261695TruSeq Adapter, Index 14 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT9550.15498994594015603Illumina PCR Primer Index 3 (95% over 21bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCATTGCGTC8860.14379171947955838No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA8810.1429802537940078No Hit
AGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT8690.14103273614868647Illumina PCR Primer Index 3 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCATT8180.1327557861560708No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCATTGCGTCTCG7680.12464112930056527No Hit
AAAGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTT7390.11993462832437203No Hit
TAGACCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCT7240.11750023126772038No Hit
GAATCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTT6980.1132806097028575TruSeq Adapter, Index 14 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA700.087.570994
CGTTTTT38700.086.924281
TAGACGG500.084.9249041
ATCACGG452.7284841E-1183.876461
GGATGGC11950.082.188427
CGGGTAT750.081.454365
AGGGATG14600.081.111035
AGTACGG700.080.880871
GCTACGA8400.080.834764
GGCGATG1050.080.559267
AAGGGCA4850.079.723634
TCCGCTA8600.079.548521
CGCTACG8550.079.416613
CGACCAA8500.078.505788
CTACGAC8700.078.32155
AGAGGGC7350.078.2685853
GAGGGTA3800.078.175724
GATGGCA11250.077.694938
AGTAAGG2250.077.5857241
TACGACC8850.076.994026
Produced by FastQC (version 0.11.5)