FastQCFastQC Report
Mon 27 Feb 2023
SRR3127436.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127436.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2715393
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT378561.3941260068063812No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT118500.086.17551
CGTAGGG8900.072.359062
TAGAGGG56550.070.406562
ATAGGGA25900.070.238233
TAAGGGA26300.069.34873
AGTAGGG48300.069.293942
AGGGATG45300.069.005835
ATAGGGC14600.067.9347463
GAATAGG18400.067.2017061
TAGGGCA13100.067.101574
ATAGAGG23250.066.731881
AGAGGGC41950.066.000273
ACTAGGG14050.065.575582
AGGGCAT23850.065.4352955
TATAGGG20600.065.2621152
AGTAAGG16800.064.926361
TAGCGGG16450.064.866772
GTACGGG6200.064.445052
AGGGTAA10750.064.2793055
GGTAAGG16700.064.189021
Produced by FastQC (version 0.11.5)