FastQCFastQC Report
Mon 27 Feb 2023
SRR3127406.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127406.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2246839
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285201.2693388355818997No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT92000.085.247071
CGTAGGG7450.078.864212
ATAGGGC12850.073.888093
TAGGGCA13500.071.026864
AGGGATG29000.070.666695
AGTAGGG36950.070.091192
GTAGGGT9150.069.862493
TAGGGTC6450.069.2293244
AGATAGG12600.069.03551
TAGGGCG4350.068.073414
TAGGGTA8500.068.016214
ATAACGG1800.067.9161
GTAGGGA16950.066.830333
AAGGGGC63250.066.65894
AGGGCAT19150.066.51615
TACGGGT1700.066.357283
ACGGGTA2200.066.2316064
GTACGGG5050.066.0835342
TAGAGGG43750.065.750582
ATGAGGG40800.065.666062
Produced by FastQC (version 0.11.5)